Lollipops In The Lab: Sweet Solution Found For Non-Intrusive Diagnostic Testing

In a delightful breakthrough, researchers have introduced a novel approach to diagnostic testing that combines the joy of lollipops with the efficiency of saliva collection.

A recent publication in ACS' Analytical Chemistry showcases the successful use of a lollipop-based saliva collection system that can capture bacteria from adults and remain shelf-stable for up to a year. Astonishingly, participants in the study not only preferred this sweet solution over traditional collection methods but also found it less invasive.

Currently, throat swabs are commonly used for sample collection in diagnosing various illnesses, including strep throat. However, this method can be discomforting and induce a gag reflex in many individuals. Alternatively, saliva sampling offers a less intrusive technique, where technicians analyze a patient's spit using methods such as quantitative polymerase chain reaction (qPCR).

The simplicity of saliva sampling has made it a popular choice for at-home testing, especially during the COVID-19 pandemic. However, gathering a sufficient amount of saliva can still be an unpleasant task. To address this issue and inject some enjoyment into the process, scientists have turned to the world of confectionery and combined it with saliva collection.

Previously, a team of researchers including Sanitta Thongpang , Ashleigh Theberge , Erwin Berthier , and their colleagues developed a unique lollipop-based collection device named CandyCollect . At first glance, CandyCollect resembles a regular lollipop , except for its spoon-like stick, which features a spiral-shaped groove carved into the top.

The flattened end of the lollipop is covered with isomalt candy, allowing saliva to effortlessly flow into the groove as the lollipop is enjoyed. In a previous laboratory study, the researchers demonstrated that this innovative device could successfully capture bacteria responsible for strep throat.

In their latest study, the researchers aimed to expand the application of CandyCollect to target other naturally occurring bacteria. They compared their system with two commercially available, at-home saliva sampling kits using real participants.

The researchers provided CandyCollect and the two conventional sampling kits to 28 adult volunteers. The participants utilized the devices, answered survey questions regarding their experience, and then sent the devices back to the lab. The researchers extracted the samples, which were later analyzed using qPCR to quantify the presence of Streptococcus mutans and Staphylococcus aureus bacteria.

Remarkably, whenever one or both of the conventional methods detected the target bacteria, CandyCollect achieved a 100 percent detection rate as well. Furthermore, among the three methods, the lollipop-based approach was the most popular choice among the participants, who also found it to be the "most sanitary" and "least disgusting."

Notably, even after being stored for a year, CandyCollect devices produced accurate results, demonstrating their durability. Although further studies are ongoing, the team believes that their work signifies the adaptability and user acceptance of the system. They anticipate that this innovative approach could inspire other scientists to develop more intuitive and convenient at-home testing methods.

In summary, the integration of a lollipop-based saliva collection system into diagnostic testing offers a unique and enjoyable alternative to traditional methods. Not only does it simplify the process for patients, but it also maintains high accuracy and long-term stability. With its success, this innovative approach paves the way for future advancements in at-home testing, ensuring convenience and user satisfaction.